Design of Self-adjustable Off-focus Positioning System with Subnano Precision



Article Abstract: The main development direction of single particle tracking technology is to improve its space and time resolution.We designed a self-adjustable off-focus positioning system based on off-focus image analysis,which efficiently increases the timeliness and accuracy of single particle tracking system and makes it possible to track single protein or vesicle in three dimensions,in whole cell,and in real time with subnanometer precision.

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Author: Wang Xiaoxuan) Yang Jundong) Hu Zhixiang) Qu Anlian)
Publisher: Institute of Biochemisty and Biophysics,School Life Science and Technology,Hust1),Wuhan 430074)(Unit 44,No.93253 Troops of PLA2),Dandong 118000
Keywords:single particle tracking, selfadjustable offfocus positioning, Zaxis displacement control